dsred gene Search Results


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Becton Dickinson rfp (dsred-2) gene
Rfp (Dsred 2) Gene, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation capsid protein gene p1 linking with the dsred gene
Capsid Protein Gene P1 Linking With The Dsred Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Sangon Biotech dsred gene sequence
Dsred Gene Sequence, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dsred gene sequence/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
dsred gene sequence - by Bioz Stars, 2026-05
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Makoto USA Inc mesorhizobium loti maff303099 carrying the dsred reporter gene
Mesorhizobium Loti Maff303099 Carrying The Dsred Reporter Gene, supplied by Makoto USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
System Biosciences Inc plasmid dna containing the gfp or dsred gene expressed by a cmv immediate-early enhancer/promoter
Experimental design of chicken C-X-C chemokine receptor type 4 ( CXCR4 ) gene targeting. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis of CXCR4 transcript in different chicken cell types (primordial germ cells [PGCs], <t>DT40,</t> and DF1). PGCs and DT40 cells strongly express CXCR4, but DF1 cells do not. (B) Schematic of CRISPR targeting of the chicken CXCR4 gene on chromosome 7 and genomic mutation analysis. Sequences of the guide RNA target site are located in the second exon (yellow box). TGG in red is a protospacer adjacent motif (PAM). Lowercase and capital letters are intron and exon sequences, respectively. Green boxes denote primer annealing sites. (C) Mutated target DNA sequences of chicken CXCR4 KO PGC line #3 induced by CRISPR/Cas9. Dashes denote deleted nucleotides. Mutant genotypes show a frameshift in translated proteins that induces amino acid changes and a stop codon (asterisk). (D) Genotyping analysis of CXCR4 RT-PCR product amplified from CXCR4 KO PGC line #3.
Plasmid Dna Containing The Gfp Or Dsred Gene Expressed By A Cmv Immediate Early Enhancer/Promoter, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna containing the gfp or dsred gene expressed by a cmv immediate-early enhancer/promoter/product/System Biosciences Inc
Average 90 stars, based on 1 article reviews
plasmid dna containing the gfp or dsred gene expressed by a cmv immediate-early enhancer/promoter - by Bioz Stars, 2026-05
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Becton Dickinson the dsred-1 gene
Experimental design of chicken C-X-C chemokine receptor type 4 ( CXCR4 ) gene targeting. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis of CXCR4 transcript in different chicken cell types (primordial germ cells [PGCs], <t>DT40,</t> and DF1). PGCs and DT40 cells strongly express CXCR4, but DF1 cells do not. (B) Schematic of CRISPR targeting of the chicken CXCR4 gene on chromosome 7 and genomic mutation analysis. Sequences of the guide RNA target site are located in the second exon (yellow box). TGG in red is a protospacer adjacent motif (PAM). Lowercase and capital letters are intron and exon sequences, respectively. Green boxes denote primer annealing sites. (C) Mutated target DNA sequences of chicken CXCR4 KO PGC line #3 induced by CRISPR/Cas9. Dashes denote deleted nucleotides. Mutant genotypes show a frameshift in translated proteins that induces amino acid changes and a stop codon (asterisk). (D) Genotyping analysis of CXCR4 RT-PCR product amplified from CXCR4 KO PGC line #3.
The Dsred 1 Gene, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/the dsred-1 gene/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
the dsred-1 gene - by Bioz Stars, 2026-05
90/100 stars
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90
Becton Dickinson dsred gene
Experimental design of chicken C-X-C chemokine receptor type 4 ( CXCR4 ) gene targeting. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis of CXCR4 transcript in different chicken cell types (primordial germ cells [PGCs], <t>DT40,</t> and DF1). PGCs and DT40 cells strongly express CXCR4, but DF1 cells do not. (B) Schematic of CRISPR targeting of the chicken CXCR4 gene on chromosome 7 and genomic mutation analysis. Sequences of the guide RNA target site are located in the second exon (yellow box). TGG in red is a protospacer adjacent motif (PAM). Lowercase and capital letters are intron and exon sequences, respectively. Green boxes denote primer annealing sites. (C) Mutated target DNA sequences of chicken CXCR4 KO PGC line #3 induced by CRISPR/Cas9. Dashes denote deleted nucleotides. Mutant genotypes show a frameshift in translated proteins that induces amino acid changes and a stop codon (asterisk). (D) Genotyping analysis of CXCR4 RT-PCR product amplified from CXCR4 KO PGC line #3.
Dsred Gene, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dsred gene/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
dsred gene - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Experimental design of chicken C-X-C chemokine receptor type 4 ( CXCR4 ) gene targeting. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis of CXCR4 transcript in different chicken cell types (primordial germ cells [PGCs], DT40, and DF1). PGCs and DT40 cells strongly express CXCR4, but DF1 cells do not. (B) Schematic of CRISPR targeting of the chicken CXCR4 gene on chromosome 7 and genomic mutation analysis. Sequences of the guide RNA target site are located in the second exon (yellow box). TGG in red is a protospacer adjacent motif (PAM). Lowercase and capital letters are intron and exon sequences, respectively. Green boxes denote primer annealing sites. (C) Mutated target DNA sequences of chicken CXCR4 KO PGC line #3 induced by CRISPR/Cas9. Dashes denote deleted nucleotides. Mutant genotypes show a frameshift in translated proteins that induces amino acid changes and a stop codon (asterisk). (D) Genotyping analysis of CXCR4 RT-PCR product amplified from CXCR4 KO PGC line #3.

Journal: The Journal of Reproduction and Development

Article Title: C-X-C chemokine receptor type 4 (CXCR4) is a key receptor for chicken primordial germ cell migration

doi: 10.1262/jrd.2017-067

Figure Lengend Snippet: Experimental design of chicken C-X-C chemokine receptor type 4 ( CXCR4 ) gene targeting. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis of CXCR4 transcript in different chicken cell types (primordial germ cells [PGCs], DT40, and DF1). PGCs and DT40 cells strongly express CXCR4, but DF1 cells do not. (B) Schematic of CRISPR targeting of the chicken CXCR4 gene on chromosome 7 and genomic mutation analysis. Sequences of the guide RNA target site are located in the second exon (yellow box). TGG in red is a protospacer adjacent motif (PAM). Lowercase and capital letters are intron and exon sequences, respectively. Green boxes denote primer annealing sites. (C) Mutated target DNA sequences of chicken CXCR4 KO PGC line #3 induced by CRISPR/Cas9. Dashes denote deleted nucleotides. Mutant genotypes show a frameshift in translated proteins that induces amino acid changes and a stop codon (asterisk). (D) Genotyping analysis of CXCR4 RT-PCR product amplified from CXCR4 KO PGC line #3.

Article Snippet: To generate GFP- or DsRed-expressing PGCs, DF1, and DT40 cells, plasmid DNA containing the GFP or DsRed gene expressed by a CMV immediate-early enhancer/promoter (System Biosciences) was transfected using Lipofectamine ® (Invitrogen) according to the manufacturer’s protocol.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, CRISPR, Mutagenesis, Amplification

Establishment of stably-transfected cells. (A) Schematic diagrams of transgene expression vectors for enhanced green fluorescent protein (eGFP), Discosoma red fluorescent protein (DsRed), and chicken CXCR4 controlled by the cytomegalovirus (CMV) promoter. Constructs were inserted between 5’-terminal repeat (5’-TR) and 3’-TR piggy Bac transposon elements. The neomycin-resistant (Neo R ) gene was used as a selection marker. (B) RT-PCR analysis after transfection and selection of piggy Bac CMV-chicken CXCR4-overexpressing (OE) DF1 cells. (C) Fluorescent images of GFP or DsRed-expressing wild type and GFP-expressing CXCR4 KO PGCs. All PGC sublines strongly expressed the fluorescent reporters (magnification, 100 ×). (D) GFP-expressing DF1 and DT40 cells after transfection and selection of the piggy Bac CMV-eGFP expression vector (magnification, 100 ×).

Journal: The Journal of Reproduction and Development

Article Title: C-X-C chemokine receptor type 4 (CXCR4) is a key receptor for chicken primordial germ cell migration

doi: 10.1262/jrd.2017-067

Figure Lengend Snippet: Establishment of stably-transfected cells. (A) Schematic diagrams of transgene expression vectors for enhanced green fluorescent protein (eGFP), Discosoma red fluorescent protein (DsRed), and chicken CXCR4 controlled by the cytomegalovirus (CMV) promoter. Constructs were inserted between 5’-terminal repeat (5’-TR) and 3’-TR piggy Bac transposon elements. The neomycin-resistant (Neo R ) gene was used as a selection marker. (B) RT-PCR analysis after transfection and selection of piggy Bac CMV-chicken CXCR4-overexpressing (OE) DF1 cells. (C) Fluorescent images of GFP or DsRed-expressing wild type and GFP-expressing CXCR4 KO PGCs. All PGC sublines strongly expressed the fluorescent reporters (magnification, 100 ×). (D) GFP-expressing DF1 and DT40 cells after transfection and selection of the piggy Bac CMV-eGFP expression vector (magnification, 100 ×).

Article Snippet: To generate GFP- or DsRed-expressing PGCs, DF1, and DT40 cells, plasmid DNA containing the GFP or DsRed gene expressed by a CMV immediate-early enhancer/promoter (System Biosciences) was transfected using Lipofectamine ® (Invitrogen) according to the manufacturer’s protocol.

Techniques: Stable Transfection, Transfection, Expressing, Construct, Selection, Marker, Reverse Transcription Polymerase Chain Reaction, Plasmid Preparation

In vivo migration assay of GFP-expressing DT40 and CXCR4 OE DF1 cells. GFP-expressing DT40 or DF1 cells carrying the CMV-controlled chicken CXCR4-overexpressing transgene were transplanted into the bloodstream of recipient embryos. Embryonic gonads were dissected on Day 6 (A) and Day 10 (B). GFP-expressing cells were examined using a fluorescent microscope.

Journal: The Journal of Reproduction and Development

Article Title: C-X-C chemokine receptor type 4 (CXCR4) is a key receptor for chicken primordial germ cell migration

doi: 10.1262/jrd.2017-067

Figure Lengend Snippet: In vivo migration assay of GFP-expressing DT40 and CXCR4 OE DF1 cells. GFP-expressing DT40 or DF1 cells carrying the CMV-controlled chicken CXCR4-overexpressing transgene were transplanted into the bloodstream of recipient embryos. Embryonic gonads were dissected on Day 6 (A) and Day 10 (B). GFP-expressing cells were examined using a fluorescent microscope.

Article Snippet: To generate GFP- or DsRed-expressing PGCs, DF1, and DT40 cells, plasmid DNA containing the GFP or DsRed gene expressed by a CMV immediate-early enhancer/promoter (System Biosciences) was transfected using Lipofectamine ® (Invitrogen) according to the manufacturer’s protocol.

Techniques: In Vivo, Migration, Expressing, Microscopy